Hydrophobic Resin

Rp20

Hydrophobic interaction chromatography (HIC) is a versatile method for the purification and separation of biomolecules by using the function of hydrophobicity. The proteins containing both hydrophilic and hydrophobic regions are applied to a HIC column under specified salt buffer conditions, which promotes the binding of the biomolecule to the HIC resin but also has a stabilizing influence on the molecule’s structure. HIC steps are commonly used at all steps in the process including the capture step, intermediate steps, and final polish purification.

Clear

Thermo Scientific™ POROS™ hydrophobic interaction chromatography (HIC) resins are based on the 50 μm POROS base bead, utilizing a novel coating procedure to enable functionalization with unique hydrophobic ligands. These resins are suitable for bind/elute and flow-through applications at lower salt concentrations and have higher binding capacity and resolution than classical HIC resins, thereby providing more flexibility around process operating conditions. Thermo Fisher Scientific provides a suite of POROS HIC resins offering a differentiating range in hydrophobicity suitable for bind/elute and flow-through applications. The resins can be used for the purification of a wide variety of biomolecules including therapeutic proteins, antibody fragments, antibody drug conjugates (ADCs), and other large molecules.

product-type

Consumable

class

Chromatography Resin

applications

Features & Benefits

Key features

• 3 unique resins covering a wide range of hydrophobicity

• High resolution, even with lower conductivity conditions

• High dynamic binding capacity and superior pressure-flow characteristics Key applications

• Monoclonal antibodies, bispecific antibodies, and antibody–drug conjugates (ADCs)

• Product-related impurities and aggregate removal

• Plasmids, RNAi, and oligonucleotides

Specifications

Official Website

Hydrophobic Resin – Official Website

Brochures

Hydrophobic Resin – Brochures